SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Native or oxidized AN (40 μM) and FN (0. 1. 12911151) 0. Molecular Devices are known for both the reliability and the wide uses of their products. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. The MPO assay was conducted according to the. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Description 1. Microplate Stacker. Description 1. Promega. Find regional support. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The drawing shows a standard microplate of 15 mm height covered with a lid. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Microplate Reader. Molecular Devices Corporation. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. SeeSpectraMax MiniMaxImagingCytometeronpage85. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. 4. 3. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The standard solutions (0. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. 0 mm hex key. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Automated for ease of use. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Then, the cell area was calculated for each. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Introduction. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Examples: fiPress carefully to engage,fl fiDo not press down. Patented user-exchangeable cartridge design expands the system's detection capability making it. 99. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. The SoftMax Pro Software provides integrated instrument control. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. To learn more about the device, please watch this short video. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. detection cartridge LUM96: 59. 00800 665 32860. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. When the user applies a set temperature of 37°C, the top is heated to 37. The dual auto injector module. 2 only) SpectraMax M-series Microplate readers. We would like to show you a description here but the site won’t allow us. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). applications, including dual luciferase and ATP assays. Remove the transport bracket and store the retaining screws in the bag that held the 3. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. SpectraMaxi3Multi. Budget-friendly multi-mode microplate reader . HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. 4. *On the SpectraMax i3x reader only. Detection of pro-inflammatory factors. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. 303 unable to cal dark current. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Art-science interactive light show/animation. 04, 0. iD5. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. XPS spectra were obtained from PHI Versaprobe II. 11. i3. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 6 in. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. edu) to be added as a trained user in iLab. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. 吸収度マイクロプレートリーダー SpectraMax® Mini. As. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Shake intensity. Table 1. techsupport. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Manufacturers try to design their instruments so they can be. The SpectraMax i2X is used for cell migration, cell. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Nucleic Acid and Protein Quantitation. . • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The treatments were. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Molecular Devicesの全商品を見る. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Pick the best speed, sensitivity, and price for your needs. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. HTRF-compatible SpectraMax Multi-Mode Readers. Ease of use. Italic and boldface type are used for emphasis. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. The plate was covered and incubated for one hour at room temperature. The chamber. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. The calculated LOQ values were 0. 0´ 104 8. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Published Dec 16, 2020 | Updated Dec 16, 2020. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Cell counters for accurate and. 2. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Microplate optimization should be performed the first time the assay is run, and subsequently for . Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Recommended settings for SpectraMax iD5 and i3x readers. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Bookings are free, but you will need to use iLab to book. This way you’ll save time on finding the necessary info. Find regional support. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. The corrected absorbance is within 5% of the value. Louis, MO) and used as received without further purification. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). 2 in. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. 2C). Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 635. Lower limits of detection for ATP determined using the ATPlite . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Explore a wealth of applications with the future-ready SpectraMax i3x. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. 3) At the bottom of the strip, click on "Show. i3x. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 6 in. ZERO BIAS - scores, article reviews, protocol conditions and more. Register to download your free eBook today. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. SpectraMax Quant dsDNA Assay Kits. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. User-upgradeable detection modules allow you to expand the capabilities of your microp. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. i3. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Data Analysis. 9 x 10-15. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Multi-Mode Reader. Bioz Stars score: 98/100, based on 1 PubMed citations. Increased fluorescence performance. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 吸収度マイクロプレートリーダー SpectraMax® Mini. Price from $9. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. It allows you to explore cellular pathways and protein activation and expression in one system. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Features. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. This way you’ll save time on finding the necessary info. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. The information below applies to a standard 96 well plate, set to a linear shake mode. Explore a wealth of applications with the future-ready SpectraMax i3x. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Settings not required by a reader are indicated by ‘---’. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. ISM Equipment Classification (Group 1, Class A) This equipment is. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. no. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Nuclear Receptor. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Download PDF. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. We would like to show you a description here but the site won’t allow us. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. 2 (Molecular Devices, San Jose, CA). Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. As. Fluorescence Microplate Reader Comparison Chart. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Open the catalog to page 1. Carbonyl assay The plates for carbonyl detection were prepared as described. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. SpectraMax i3x Multi-Mode Detection Platform. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Reporter Assay. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. 1321N1, a human astrocytoma cell line. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Italic and boldface type are used for emphasis. All test measurements and calculations are handlediD5. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. See SpectraMax® Microplate Reader User Manual Download Page. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. ZERO BIAS - scores, article reviews, protocol conditions and more. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. 660-665 Eskdale Road. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax i3x and Paradigm readers require the FP-FLUO. Controls containing all reaction components with either DI . User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Features. Average 98 stars, based on 1 article reviews. It allows you to explore cellular pathways and protein activation and expression in one system. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Figure 3. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Prepare your i3/i3x instrument for shipment. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 312 gain calibration failed. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Each heating element is independently. Molecular Devicesの全商品を見る. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. In this product video, see how the SpectraMax M5 quickly reads samples. 200-800 nm using Spectra Max i3X microplate reader. 제품. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The assay window spanned four orders of magnitude. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Contact Local Team. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. A mixture of 6-nitro-1H,3H-benzo[de]isochromene-1,3-dione (486 mg, 2. In addition, Image J was used to quantify the. 315 can't find zero order. HTRF IP standard curve. 800. It allows you to explore cellular . The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Multimode Reader Comparison Chart. It allows you to explore cellular pathways and protein activation and expression in one system. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 1. It allows you to explore cellular . The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Molecular Devices Software. Prepare your i3/i3x instrument for shipment. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The size and fluorescence intensity were tested for 48 h. A: percent GFP-positive cells calculated using the classification feature in the software. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. For wavelengths in the UV range above 220 nm,. The SpectraMax ® i3x reader provides the. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Sunnyvale, CA, USA) with excitation at 475 nm. 001 0. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Prepare your iD series instrument for shipment. Order Now. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 3) At the bottom of the strip, click on "Show. Spectramax I3x, supplied by Danaher Inc, used in various techniques. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. Examples: fiPress carefully to engage,fl fiDo not press down. Materials and methodsWe would like to show you a description here but the site won’t allow us. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. 0´ 104 6. Italic and boldface type are used for emphasis. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. How to properly unlock iD series instrument. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. , 1997). com. TUNE Tunable Wavelength Detection Cartridge. Prime function 20 amol. Get Pricing. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. ) deep, preferably with access on all sides. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Pick the best speed, sensitivity, and price for your needs. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. 306 plate air cal. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 1. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. . Microplate reader assays requiring infrared (IR) or red-shifted. The treatments were. ) deep, preferably with access on all sides. Thetypeof. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Anne T. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. We would like to show you a description here but the site won’t allow us. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. , 2021).